Jungle and Drum and bass did use the Amen break a lot, as well as the Funky Drummer and a handful of others. These days though, most drums in Drum and Bass music are made by stacking individual percussion samples (like a bunch of snares, a couple of kick drums, etc.). The Amen break is still used heavily, but often with a thick filter over it just for the purpose of filling the drums out a bit more.
That said, the Amen break is used a lot outside of Drum and bass as well. It’s like the Wilhelm scream of the music industry.