Or come up with a less simplistic means of helping people through it. And Biden actually would. Trump will promise to, then once he has no re-election fears, just go back on the promise completely.
7 Likes
Iâm only interested in ingesting home brew alcohol.
7 Likes
He has the special power to do things and not have the entire weight of the media, the opposition and half his own party pile on him. But I hope Biden does stuff like this anyway.
4 Likes
Both will resume sacrificing people at the temple of Mammon sooner or later, just a matter of when
6 Likes
The scope of this problem is way too big to let go. We canât have millions of homeless families dumped on the street all at once. I mean, granted, big urban areas are blue, more often than not, so Trump probably would be happy with that, but still, I think the riots now would pale in comparison.
After, though, yeah. Send in the lobbyistsâŚ
5 Likes
They keep changing which peptides theyâre using based on new results. In particular, theyâre trying to switch to targeting highly conserved epitopes to minimize the likelihood of mutant escape, and avoid epitopes with a strong CD4+ T-cell response until the concerns about CD4+ hyperstimulation and cytokine storm are resolved. They are also trying to make sure that epitopes that elicit a B-cell response are ones where the antibody would impede the function of the virus, to avoid the possibility of antibody-dependent enhancement.
There was an update on 21 August to the whitepaper giving the sequences theyâre currently using.
Their Gen-8 vaccine is 19-valent: 12 of the 19 epitopes are from Spike. Others are from the nucleocapsid N protein, Orf1, ORF1ab, and Nsp7. Several have amino acid substitutions to improve solubility, to improve association with the chitosan adjuvant, and/or additional Cys residues so that they can be circularized to be more stable and present something like the native conformation (important for B cell response). Each peptide requires only 3-4 micrograms per dose, but with the list being so long, this looks like an expensive proposition if you donât already work in a lab tooled for it.
If a lab is tooled for it, this looks like an easy enough formulation that any number of contract labs could scale it up. If it comes to guerilla vaccine production, Iâm starting to think that the guerrilla fighters might be out there!
6 Likes
You go for it! My lab technique is way not up to this kinda synthesis. Iâll stick to âsynthesizingâ really excellent mead!
13 Likes
As expected:
19 Likes
Until 45, there were a lot of things I thought could never happen in the US. Now, I wouldnât put anything past the current administration. Theyâve proven over and over again that they do not care about the health and well-being of anyone except their inner circle of friends and supporters.
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12 Likes
Iâm far from being tooled for the solid-state peptide synthesis. Charcoal circularization practically runs itself, though, and the final formulation is dead easy - everythingâs water soluble, itâs just pipetting, mixing, heating, cooling - but to get to that point, you need the peptides. (And someone needs to have run the HPLC and mass spec for quality!)
The contract labs all have automated synthesis machines that can turn out these short sequences. Some of the contract labs can turn out peptides in multi-kilogram lots. That translates to âenough vaccine to immunize the whole USâ in one run per peptide, since weâre talking about a few micrograms per dose. All the rest of the stuff thatâs needed is industrial chemicals that you can order by the truckload.
In short, I expect continued fuckery from the government, and I expect that in the face of the fuckery, there may well be widespread availability of an illegal but effective vaccine. In the current political climate, Iâm willing to accept a peer-reviewed medium-scale trial in lieu of a regulated Phase 3. The government fuckery is going to change fundamentally the way clinical science gets done.
At least with this stuff, all the ingredients except the peptides themselves have been through clinical trials and have low toxicity. Weâll have to see what comes out of running immunoassays on the nasal wash results from the team, to have any idea about whether itâs eliciting a mucosal response. although the fact that the booster elicits brief head-cold symptoms is very promising, indeed.
I think that the Northeastern states would be willing to immunize with gray-market vaccine if it comes to that. A couple have already resorted to smuggling PPE to prevent Uncle Sam from stealing it. The national authorities have been de-legitimized to the point where weâre breaking entirely new ground here. Fortunately for us, there are a lot of med-chem labs in New Jersey!
Big drawback - the platform is likely to require one or more boosters at intervals on the order of a couple of weeks. Mitigating that - a patient can self-administer an intranasal vaccine; itâs no harder than snorting Flonase.
6 Likes
Iâll take the risk. My essence has been too pure for far too long.
11 Likes
56 year old man thinks heâs young. A mere yute.
13 Likes
Thatâs the Dr. Drew influence.
7 Likes
But heâs 61.
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I was thinking more of his understanding of covid.
7 Likes
May karma kick his ass, and soon.
16 Likes
Election Gas LightingâŚ
Any vaccine before Nov should scare the hell out of everybody.
What is it CDC?
CDC: âBleach. You gotta a problem with that?â
20 Likes
Yeah⌠until Canadaâs gov or somewhere thatâs still reputable says a vaccine is safe for the masses, all that BS coming from the corrupted CDC can miss me.
16 Likes
Donât even want to know about his views on China and the virus.
Or anything. Ever. Like, âOh god, Iâm on fire, Iâm burning, someone help me!â
Shut it, asshole. Talk to the hand.
âNow get it up inside there, somehow.â
10 Likes
